A Selection and Analysis Tool for Fluorescence Recovery after Photobleaching (FRAP) Region of Interest (ROI)

Initializing live version
Download to Desktop

Requires a Wolfram Notebook System

Interact on desktop, mobile and cloud with the free Wolfram Player or other Wolfram Language products.

Fluorescence recovery after photobleaching (FRAP) can be used to quantify the mobility of molecules in live cells. In a typical FRAP experiment, a portion of a cell expressing a fluorescently tagged molecule of interest is photobleached and the recovery of the photobleached region is monitored as a function of time. Briefly, faster recoveries correspond to more mobile molecules. In this Demonstration a movie of a FRAP experiment is analyzed. You can scroll through the movie in the upper display and choose the size and position of a circular region of interest (ROI). The normalized intensity of the fluorescence in the ROI is displayed as a function of image number in a plot below. A vertical red line designates the current image number of the movie in the upper display.

Contributed by: Timothy J. Stasevich (April 2011)
Open content licensed under CC BY-NC-SA


Snapshots


Details

For details on FRAP, see the Wikipedia article "Fluorescence Recovery after Photobleaching".



Feedback (field required)
Email (field required) Name
Occupation Organization
Note: Your message & contact information may be shared with the author of any specific Demonstration for which you give feedback.
Send