Liquid Chromatography Simulation

The chromatographic column is modeled as a first-in, last-out shift register in which each element of the register is a "plate". Exchange processes between the mobile and stationary phases are independently calculated in each plate during a shift cycle. The column dimensions are fixed but undefined. Component amounts are arbitrary. Time units are equivalent to plate residence times, determined by the number of plates and transit time. You have the option of altering the time scale, but the axis is automatically adjusted to the maximum exit concentration.

Snapshot 1: adsorption model normal plot

Snapshot 2: partition model cumulative output plot

Snapshot 3: potential for mass separation with input width much greater than 1

Snapshot 4: component 2 exponentially modified partition model

= initial component amount/plate entering column ,

= equilibrium constant ratio of effective mobile to stationary phase volumes = Langmuir equilibrium constant / effective mobile phase volume

= adsorption component saturation level/plate

= first order exponential modification constant

For more information, see: T. W. Shattuck, "HPL Simulator".